Insectivorous Plants Part 3

It is an important fact that when an organic or inorganic object is placed on the glands of the disc, and the exterior tentacles are thus caused to bend inwards, not only is the secretion from the glands of the latter increased in quant.i.ty and rendered acid, but the contents of the cells of their pedicels become aggregated. The process always commences in the glands, although these have not as yet touched any object. Some force or influence must, therefore, be transmitted from the central glands to the exterior tentacles, first to near their bases causing this part to bend, and next to the glands causing them to secrete more copiously. After a short time the glands, thus indirectly excited, transmit or reflect some influence down their own pedicels, inducing aggregation in cell beneath cell to their bases.

It seems at first sight a probable view that aggregation is due to the glands being excited to secrete more copiously, so that sufficient fluid is not left in their cells, and in the cells of the pedicels, to hold the protoplasm in solution. In favour of this view is the fact that aggregation follows the inflection of the tentacles, and during the movement the glands generally, or, as I believe, always, secrete more copiously than they did before. Again, during the re-expansion [page 56] of the tentacles, the glands secrete less freely, or quite cease to secrete, and the aggregated ma.s.ses of protoplasm are then redissolved. Moreover, when leaves are immersed in dense vegetable solutions, or in glycerine, the fluid within the gland-cells pa.s.ses outwards, and there is aggregation; and when the leaves are afterwards immersed in water, or in an innocuous fluid of less specific gravity than water, the protoplasm is redissolved, and this, no doubt, is due to endosmose.

Opposed to this view, that aggregation is caused by the outward pa.s.sage of fluid from the cells, are the following facts. There seems no close relation between the degree of increased secretion and that of aggregation. Thus a particle of sugar added to the secretion round a gland causes a much greater increase of secretion, and much less aggregation, than does a particle of carbonate of ammonia given in the same manner. It does not appear probable that pure water would cause much exosmose, and yet aggregation often follows from an immersion in water of between 16 hrs. and 24 hrs., and always after from 24 hrs. to 48 hrs. Still less probable is it that water at a temperature of from 125o to 130o Fahr. (51o.6 to 54o.4 Cent.) should cause fluid to pa.s.s, not only from the glands, but from all the cells of the tentacles down to their bases, so quickly that aggregation is induced within 2 m. or 3 m. Another strong argument against this view is, that, after complete aggregation, the spheres and oval ma.s.ses of protoplasm float about in an abundant supply of thin colourless fluid; so that at least the latter stages of the process cannot be due to the want of fluid to hold the protoplasm in solution. There is still stronger evidence that aggregation is independent of secretion; for the papillae, described in the first chapter, with which the [page 57] leaves are studded are not glandular, and do not secrete, yet they rapidly absorb carbonate of ammonia or an infusion of raw meat, and their contents then quickly undergo aggregation, which afterwards spreads into the cells of the surrounding tissues. We shall hereafter see that the purple fluid within the sensitive filaments of Dionaea, which do not secrete, likewise undergoes aggregation from the action of a weak solution of carbonate of ammonia.

The process of aggregation is a vital one; by which I mean that the contents of the cells must be alive and uninjured to be thus affected, and they must be in an oxygenated condition for the transmission of the process at the proper rate. Some tentacles in a drop of water were strongly pressed beneath a slip of gla.s.s; many of the cells were ruptured, and pulpy matter of a purple colour, with granules of all sizes and shapes, exuded, but hardly any of the cells were completely emptied. I then added a minute drop of a solution of one part of carbonate of ammonia to 109 of water, and after 1 hr. examined the specimens. Here and there a few cells, both in the glands and in the pedicels, had escaped being ruptured, and their contents were well aggregated into spheres which were constantly changing their forms and positions, and a current could still be seen flowing along the walls; so that the protoplasm was alive. On the other hand, the exuded matter, which was now almost colourless instead of being purple, did not exhibit a trace of aggregation. Nor was there a trace in the many cells which were ruptured, but which had not been completely emptied of their contents. Though I looked carefully, no signs of a current could be seen within these ruptured cells. They had evidently been killed by the pressure; and the matter which they [page 58] still contained did not undergo aggregation any more than that which had exuded. In these specimens, as I may add, the individuality of the life of each cell was well ill.u.s.trated.

A full account will be given in the next chapter of the effects of heat on the leaves, and I need here only state that leaves immersed for a short time in water at a temperature of 120oFahr. (48o.8 Cent.), which, as we have seen, does not immediately induce aggregation, were then placed in a few drops of a strong solution of one part of carbonate of ammonia to 109 of water, and became finely aggregated. On the other hand, leaves, after an immersion in water at 150o (65o.5 Cent.), on being placed in the same strong solution, did not undergo aggregation, the cells becoming filled with brownish, pulpy, or muddy matter. With leaves subjected to temperatures between these two extremes of 120o and 150o Fahr. (48o.8 and 65o.5 Cent.), there were gradations in the completeness of the process; the former temperature not preventing aggregation from the subsequent action of carbonate of ammonia, the latter quite stopping it. Thus, leaves immersed in water, heated to 130o (54o.4 Cent.), and then in the solution, formed perfectly defined spheres, but these were decidedly smaller than in ordinary cases. With other leaves heated to 140o (60o Cent.), the spheres were extremely small, yet well defined, but many of the cells contained, in addition, some brownish pulpy matter. In two cases of leaves heated to 145o (62o.7 Cent.), a few tentacles could be found with some of their cells containing a few minute spheres; whilst the other cells and other whole tentacles included only the brownish, disintegrated or pulpy matter.

The fluid within the cells of the tentacles must be in an oxygenated condition, in order that the force or [page 59] influence which induces aggregation should be transmitted at the proper rate from cell to cell.

A plant, with its roots in water, was left for 45 m. in a vessel containing 122 oz. of carbonic acid. A leaf from this plant, and, for comparison, one from a fresh plant, were both immersed for 1 hr. in a rather strong solution of carbonate of ammonia. They were then compared, and certainly there was much less aggregation in the leaf which had been subjected to the carbonic acid than in the other.

Another plant was exposed in the same vessel for 2 hrs. to carbonic acid, and one of its leaves was then placed in a solution of one part of the carbonate to 437 of water; the glands were instantly blackened, showing that they had absorbed, and that their contents were aggregated; but in the cells close beneath the glands there was no aggregation even after an interval of 3 hrs. After 4 hrs. 15 m. a few minute spheres of protoplasm were formed in these cells, but even after 5 hrs. 30 m. the aggregation did not extend down the pedicels for a length equal to that of the glands. After numberless trials with fresh leaves immersed in a solution of this strength, I have never seen the aggregating action transmitted at nearly so slow a rate. Another plant was left for 2 hrs. in carbonic acid, but was then exposed for 20 m. to the open air, during which time the leaves, being of a red colour, would have absorbed some oxygen. One of them, as well as a fresh leaf for comparison, were now immersed in the same solution as before. The former were looked at repeatedly, and after an interval of 65 m. a few spheres of protoplasm were first observed in the cells close beneath the glands, but only in two or three of the longer tentacles. After 3 hrs. the aggregation had travelled down the pedicels of a few of the tentacles [page 60] for a length equal to that of the glands. On the other hand, in the fresh leaf similarly treated, aggregation was plain in many of the tentacles after 15 m.; after 65 m. it had extended down the pedicels for four, five, or more times the lengths of the glands; and after 3 hrs. the cells of all the tentacles were affected for one-third or one-half of their entire lengths. Hence there can be no doubt that the exposure of leaves to carbonic acid either stops for a time the process of aggregation, or checks the transmission of the proper influence when the glands are subsequently excited by carbonate of ammonia; and this substance acts more promptly and energetically than any other. It is known that the protoplasm of plants exhibits its spontaneous movements only as long as it is in an oxygenated condition; and so it is with the white corpuscles of the blood, only as long as they receive oxygen from the red corpuscles;* but the cases above given are somewhat different, as they relate to the delay in the generation or aggregation of the ma.s.ses of protoplasm by the exclusion of oxygen.

Summary and Concluding Remarks.--The process of aggregation is independent of the inflection of the tentacles and of increased secretion from the glands. It commences within the glands, whether these have been directly excited, or indirectly by a stimulus received from other glands. In both cases the process is transmitted from cell to cell down the whole length of the tentacles, being arrested for a short time at each transverse part.i.tion. With pale-coloured leaves the first change which is perceptible, but only

* With respect to plants, Sachs, 'Trait de Bot.' 3rd edit., 1874, p.

864. On blood corpuscles, see 'Quarterly Journal of Microscopical Science,' April 1874, p. 185.' [page 61]

under a high power, is the appearance of the finest granules in the fluid within the cells, making it slightly cloudy. These granules soon aggregate into small globular ma.s.ses. I have seen a cloud of this kind appear in 10 s. after a drop of a solution of carbonate of ammonia had been given to a gland. With dark red leaves the first visible change often is the conversion of the outer layer of the fluid within the cells into bag-like ma.s.ses. The aggregated ma.s.ses, however they may have been developed, incessantly change their forms and positions. They are not filled with fluid, but are solid to their centres. Ultimately the colourless granules in the protoplasm which flows round the walls coalesce with the central spheres or ma.s.ses; but there is still a current of limpid fluid flowing within the cells. As soon as the tentacles fully re-expand, the aggregated ma.s.ses are redissolved, and the cells become filled with h.o.m.ogeneous purple fluid, as they were at first. The process of redissolution commences at the bases of the tentacles, thence proceeding upwards to the glands; and, therefore, in a reversed direction to that of aggregation.

Aggregation is excited by the most diversified causes,--by the glands being several times touched,--by the pressure of particles of any kind, and as these are supported by the dense secretion, they can hardly press on the glands with the weight of a millionth of a grain,*--by the tentacles being cut off close beneath

* According to Hofmeister (as quoted by Sachs, 'Trait de Bot.' 1874, p.

958), very slight pressure on the cell-membrane arrests immediately the movements of the protoplasm, and even determines its separation from the walls. But the process of aggregation is a different phenomenon, as it relates to the contents of the cells, and only secondarily to the layer of protoplasm which flows along the walls; though no doubt the effects of pressure or of a touch on the outside must be transmitted through this layer. [page 62]

the glands,--by the glands absorbing various fluids or matter dissolved out of certain bodies,--by exosmose,--and by a certain degree of heat.

On the other hand, a temperature of about 150o Fahr. (65o.5 Cent.) does not excite aggregation; nor does the sudden crus.h.i.+ng of a gland. If a cell is ruptured, neither the exuded matter nor that which still remains within the cell undergoes aggregation when carbonate of ammonia is added. A very strong solution of this salt and rather large bits of raw meat prevent the aggregated ma.s.ses being well developed. From these facts we may conclude that the protoplasmic fluid within a cell does not become aggregated unless it be in a living state, and only imperfectly if the cell has been injured. We have also seen that the fluid must be in an oxygenated state, in order that the process of aggregation should travel from cell to cell at the proper rate.

Various nitrogenous organic fluids and salts of ammonia induce aggregation, but in different degrees and at very different rates.

Carbonate of ammonia is the most powerful of all known substances; the absorption of 1/134400 of a grain (.000482 mg.) by a gland suffices to cause all the cells of the same tentacle to become aggregated. The first effect of the carbonate and of certain other salts of ammonia, as well as of some other fluids, is the darkening or blackening of the glands. This follows even from long immersion in cold distilled water.

It apparently depends in chief part on the strong aggregation of their cell-contents, which thus become opaque, and do not reflect light. Some other fluids render the glands of a brighter red; whilst certain acids, though much diluted, the poison of the cobra-snake, &c., make the glands perfectly white and opaque; and this seems to depend on the coagulation of their contents without [page 63] any aggregation.

Nevertheless, before being thus affected, they are able, at least in some cases, to excite aggregation in their own tentacles.

That the central glands, if irritated, send centrifugally some influence to the exterior glands, causing them to send back a centripetal influence inducing aggregation, is perhaps the most interesting fact given in this chapter. But the whole process of aggregation is in itself a striking phenomenon. Whenever the peripheral extremity of a nerve is touched or pressed, and a sensation is felt, it is believed that an invisible molecular change is sent from one end of the nerve to the other; but when a gland of Drosera is repeatedly touched or gently pressed, we can actually see a molecular change proceeding from the gland down the tentacle; though this change is probably of a very different nature from that in a nerve. Finally, as so many and such widely different causes excite aggregation, it would appear that the living matter within the gland-cells is in so unstable a condition that almost any disturbance suffices to change its molecular nature, as in the case of certain chemical compounds. And this change in the glands, whether excited directly, or indirectly by a stimulus received from other glands, is transmitted from cell to cell, causing granules of protoplasm either to be actually generated in the previously limpid fluid or to coalesce and thus to become visible.

Supplementary Observations on the Process of Aggregation in the Roots of Plants.

It will hereafter be seen that a weak solution of the carbonate of ammonia induces aggregation in the cells of the roots of Drosera; and this led me to make a few trials on the roots of other plants. I dug up in the latter part of October the first weed which I met with, viz.

Euphorbia peplus, being care- [page 64] ful not to injure the roots; these were washed and placed in a little solution of one part of carbonate of ammonia to 146 of water. In less than one minute I saw a cloud travelling from cell to cell up the roots, with wonderful rapidity. After from 8 m. to 9 m. the fine granules, which caused this cloudy appearance, became aggregated towards the extremities of the roots into quadrangular ma.s.ses of brown matter; and some of these soon changed their forms and became spherical. Some of the cells, however, remained unaffected. I repeated the experiment with another plant of the same species, but before I could get the specimen into focus under the microscope, clouds of granules and quadrangular ma.s.ses of reddish and brown matter were formed, and had run far up all the roots. A fresh root was now left for 18 hrs. in a drachm of a solution of one part of the carbonate to 437 of water, so that it received 1/8 of a grain, or 2.024 mg. When examined, the cells of all the roots throughout their whole length contained aggregated ma.s.ses of reddish and brown matter.

Before making these experiments, several roots were closely examined, and not a trace of the cloudy appearance or of the granular ma.s.ses could be seen in any of them. Roots were also immersed for 35 m. in a solution of one part of carbonate of potash to 218 of water; but this salt produced no effect.

I may here add that thin slices of the stem of the Euphorbia were placed in the same solution, and the cells which were green instantly became cloudy, whilst others which were before colourless were clouded with brown, owing to the formation of numerous granules of this tint. I have also seen with various kinds of leaves, left for some time in a solution of carbonate of ammonia, that the grains of chlorophyll ran together and partially coalesced; and this seems to be a form of aggregation.

Plants of duck-weed (Lemna) were left for between 30 m. and 45 m. in a solution of one part of this same salt to 146 of water, and three of their roots were then examined. In two of them, all the cells which had previously contained only limpid fluid now included little green spheres. After from 1 1/2 hr. to 2 hrs. similar spheres appeared in the cells on the borders of the leaves; but whether the ammonia had travelled up the roots or had been directly absorbed by the leaves, I cannot say. As one species, Lemna arrhiza, produces no roots, the latter alternative is perhaps the most probable. After about 2 1/2 hrs.

some of the little green spheres in the roots were broken up into small granules which exhibited Brownian movements. Some duck-weed was also left for 1 hr. 30 m. in a solution of one part of [page 65] carbonate of potash to 218 of water, and no decided change could be perceived in the cells of the roots; but when these same roots were placed for 25 m.

in a solution of carbonate of ammonia of the same strength, little green spheres were formed.

A green marine alga was left for some time in this same solution, but was very doubtfully affected. On the other hand, a red marine alga, with finely pinnated fronds, was strongly affected. The contents of the cells aggregated themselves into broken rings, still of a red colour, which very slowly and slightly changed their shapes, and the central s.p.a.ces within these rings became cloudy with red granular matter. The facts here given (whether they are new, I know not) indicate that interesting results would perhaps be gained by observing the action of various saline solutions and other fluids on the roots of plants.

[page 66]

CHAPTER IV.

THE EFFECTS OF HEAT ON THE LEAVES.

Nature of the experiments--Effects of boiling water--Warm water causes rapid inflection-- Water at a higher temperature does not cause immediate inflection, but does not kill the leaves, as shown by their subsequent re-expansion and by the aggregation of the protoplasm-- A still higher temperature kills the leaves and coagulates the alb.u.minous contents of the glands.

IN my observations on Drosera rotundifolia, the leaves seemed to be more quickly inflected over animal substances, and to remain inflected for a longer period during very warm than during cold weather. I wished, therefore, to ascertain whether heat alone would induce inflection, and what temperature was the most efficient. Another interesting point presented itself, namely, at what degree life was extinguished; for Drosera offers unusual facilities in this respect, not in the loss of the power of inflection, but in that of subsequent re-expansion, and more especially in the failure of the protoplasm to become aggregated, when the leaves after being heated are immersed in a solution of carbonate of ammonia.*

* When my experiments on the effects of heat were made, I was not aware that the subject had been carefully investigated by several observers.

For instance, Sachs is convinced ('Trait de Botanique,' 1874, pp. 772, 854) that the most different kinds of plants all perish if kept for 10 m. in water at 45o to 46o Cent., or 113o to 115o Fahr.; and he concludes that the protoplasm within their cells always coagulates, if in a damp condition, at a temperature of between 50oand 60o Cent., or 122o to 140o Fahr. Max Schultze and Khne (as quoted by Dr. Bastian in 'Contemp. Review,' 1874, p. 528) "found that the protoplasm of plant-cells, with which they experimented, was always killed and [[page 67]] altered by a very brief exposure to a temperature of 118 1/2o Fahr. as a maximum." As my results are deduced from special phenomena, namely, the subsequent aggregation of the protoplasm and the re-expansion of the tentacles, they seem to me worth giving. We shall find that Drosera resists heat somewhat better than most other plants.

That there should be considerable differences in this respect is not surprising, considering that some low vegetable organisms grow in hot springs--cases of which have been collected by Prof. Wyman ('American Journal of Science,' vol. xliv. 1867). Thus, Dr. Hooker found Confervae in water at 168o Fahr.; Humboldt, at 185o Fahr.; and Descloizeaux, at 208o Fahr.) [page 67]

[My experiments were tried in the following manner. Leaves were cut off, and this does not in the least interfere with their powers; for instance, three cut off leaves, with bits of meat placed on them, were kept in a damp atmosphere, and after 23 hrs. closely embraced the meat both with their tentacles and blades; and the protoplasm within their cells was well aggregated. Three ounces of doubly distilled water was heated in a porcelain vessel, with a delicate thermometer having a long bulb obliquely suspended in it. The water was gradually raised to the required temperature by a spirit-lamp moved about under the vessel; and in all cases the leaves were continually waved for some minutes close to the bulb. They were then placed in cold water, or in a solution of carbonate of ammonia. In other cases they were left in the water, which had been raised to a certain temperature, until it cooled. Again in other cases the leaves were suddenly plunged into water of a certain temperature, and kept there for a specified time. Considering that the tentacles are extremely delicate, and that their coats are very thin, it seems scarcely possible that the fluid contents of their cells should not have been heated to within a degree or two of the temperature of the surrounding water. Any further precautions would, I think, have been superfluous, as the leaves from age or const.i.tutional causes differ slightly in their sensitiveness to heat.

It will be convenient first briefly to describe the effects of immersion for thirty seconds in boiling water. The leaves are rendered flaccid, with their tentacles bowed backwards, which, as we shall see in a future chapter, is probably due to their outer surfaces retaining their elasticity for a longer period than their inner surfaces retain the power of contraction. The purple fluid within the cells of the pedicels is rendered finely granular, but there is no true aggregation; nor does this follow [page 68] when the leaves are subsequently placed in a solution of carbonate of ammonia. But the most remarkable change is that the glands become opaque and uniformly white; and this may be attributed to the coagulation of their alb.u.minous contents.

My first and preliminary experiment consisted in putting seven leaves in the same vessel of water, and warming it slowly up to the temperature of 110o Fahr. (43o.3 Cent.); a leaf being taken out as soon as the temperature rose to 80o (26o.6 Cent.), another at 85o, another at 90o, and so on. Each leaf, when taken out, was placed in water at the temperature of my room, and the tentacles of all soon became slightly, though irregularly, inflected. They were now removed from the cold water and kept in damp air, with bits of meat placed on their discs. The leaf which had been exposed to the temperature of 110o became in 15 m. greatly inflected; and in 2 hrs. every single tentacle closely embraced the meat. So it was, but after rather longer intervals, with the six other leaves. It appears, therefore, that the warm bath had increased their sensitiveness when excited by meat.

I next observed the degree of inflection which leaves underwent within stated periods, whilst still immersed in warm water, kept as nearly as possible at the same temperature; but I will here and elsewhere give only a few of the many trials made. A leaf was left for 10 m. in water at 100o (37o.7 Cent.), but no inflection occurred. A second leaf, however, treated in the same manner, had a few of its exterior tentacles very slightly inflected in 6 m., and several irregularly but not closely inflected in 10 m. A third leaf, kept in water at 105o to 106o (40o.5 to 41o.1 Cent.), was very moderately inflected in 6 m. A fourth leaf, in water at 110o (43o.3 Cent.), was somewhat inflected in 4 m., and considerably so in from 6 to 7 m.

Three leaves were placed in water which was heated rather quickly, and by the time the temperature rose to 115o-116o (46o.1 to 46o.06 Cent.), all three were inflected. I then removed the lamp, and in a few minutes every single tentacle was closely inflected. The protoplasm within the cells was not killed, for it was seen to be in distinct movement; and the leaves, having been left in cold water for 20 hrs., re-expanded.

Another leaf was immersed in water at 100o (37.7o Cent.), which was raised to 120o (48o.8 Cent.); and all the tentacles, except the extreme marginal ones, soon became closely inflected. The leaf was now placed in cold water, and in 7 hrs. 30 m. it had partly, and in 10 hrs. fully, re-expanded. On the following morning it was immersed in a weak solution of carbonate of [page 69] ammonia, and the glands quickly became black, with strongly marked aggregation in the tentacles, showing that the protoplasm was alive, and that the glands had not lost their power of absorption. Another leaf was placed in water at 110o (43o.3 Cent.) which was raised to 120o (48o.8 Cent.); and every tentacle, excepting one, was quickly and closely inflected. This leaf was now immersed in a few drops of a strong solution of carbonate of ammonia (one part to 109 of water); in 10 m. all the glands became intensely black, and in 2 hrs. the protoplasm in the cells of the pedicels was well aggregated. Another leaf was suddenly plunged, and as usual waved about, in water at 120o, and the tentacles became inflected in from 2 m. to 3 m., but only so as to stand at right angles to the disc. The leaf was now placed in the same solution (viz. one part of carbonate of ammonia to 109 of water, or 4 grs. to 1 oz., which I will for the future designate as the strong solution), and when I looked at it again after the interval of an hour, the glands were blackened, and there was well-marked aggregation. After an additional interval of 4 hrs. the tentacles had become much more inflected. It deserves notice that a solution as strong as this never causes inflection in ordinary cases. Lastly a leaf was suddenly placed in water at 125o (51o.6 Cent.), and was left in it until the water cooled; the tentacles were rendered of a bright red and soon became inflected. The contents of the cells underwent some degree of aggregation, which in the course of three hours increased; but the ma.s.ses of protoplasm did not become spherical, as almost always occurs with leaves immersed in a solution of carbonate of ammonia.]

We learn from these cases that a temperature of from 120o to 125o (48o.8 to 51o.6 Cent.) excites the tentacles into quick movement, but does not kill the leaves, as shown either by their subsequent re-expansion or by the aggregation of the protoplasm. We shall now see that a temperature of 130o (54o.4 Cent.) is too high to cause immediate inflection, yet does not kill the leaves.

[Experiment 1.--A leaf was plunged, and as in all cases waved about for a few minutes, in water at 130o (54o.4 Cent.), but there was no trace of inflection; it was then placed in cold water, and after an interval of 15 m. very slow movement was [page 70] distinctly seen in a small ma.s.s of protoplasm in one of the cells of a tentacle.* After a few hours all the tentacles and the blade became inflected.

Experiment 2.--Another leaf was plunged into water at 130o to 131o, and as before there was no inflection. After being kept in cold water for an hour, it was placed in the strong solution of ammonia, and in the course of 55 m. the tentacles were considerably inflected. The glands, which before had been rendered of a brighter red, were now blackened.

The protoplasm in the cells of the tentacles was distinctly aggregated; but the spheres were much smaller than those generated in unheated leaves when subjected to carbonate of ammonia. After an additional 2 hrs. all the tentacles, excepting six or seven, were closely inflected.

Experiment 3.--A similar experiment to the last, with exactly the same results.

Experiment 4.--A fine leaf was placed in water at 100o (37o.7 Cent.), which was then raised to 145o (62o.7 Cent.). Soon after immersion, there was, as might have been expected, strong inflection. The leaf was now removed and left in cold water; but from having been exposed to so high a temperature, it never re-expanded.

Experiment 5.--Leaf immersed at 130o (54o.4 Cent.), and the water raised to 145o (62o.7 Cent.), there was no immediate inflection; it was then placed in cold water, and after 1 hr. 20 m. some of the tentacles on one side became inflected. This leaf was now placed in the strong solution, and in 40 m. all the submarginal tentacles were well inflected, and the glands blackened. After an additional interval of 2 hrs. 45 m. all the tentacles, except eight or ten, were closely inflected, with their cells exhibiting a slight degree of aggregation; but the spheres of protoplasm were very small, and the cells of the exterior tentacles contained some pulpy or disintegrated brownish matter.

Experiments 6 and 7.--Two leaves were plunged in water at 135o (57o.2 Cent.) which was raised to 145o (62o.7 Cent.); neither became inflected. One of these, however, after having been left for 31 m. in cold water, exhibited some slight inflection, which increased after an additional interval of 1 hr. 45 m., until

* Sachs states ('Trait de Botanique,' 1874, p. 855) that the movements of the protoplasm in the hairs of a Cucurbita ceased after they were exposed for 1 m. in water to a temperature of 47o to 48o Cent., or 117o to 119o Fahr. [page 71]

all the tentacles, except sixteen or seventeen, were more or less inflected; but the leaf was so much injured that it never re-expanded.

The other leaf, after having been left for half an hour in cold water, was put into the strong solution, but no inflection ensued; the glands, however, were blackened, and in some cells there was a little aggregation, the spheres of protoplasm being extremely small; in other cells, especially in the exterior tentacles, there was much greenish-brown pulpy matter.

Experiment 8.--A leaf was plunged and waved about for a few minutes in water at 140o (60oCent.), and was then left for half an hour in cold water, but there was no inflection. It was now placed in the strong solution, and after 2 hrs. 30 m. the inner submarginal tentacles were well inflected, with their glands blackened, and some imperfect aggregation in the cells of the pedicels. Three or four of the glands were spotted with the white porcelain-like structure, like that produced by boiling water. I have seen this result in no other instance after an immersion of only a few minutes in water at so low a temperature as 140o, and in only one leaf out of four, after a similar immersion at a temperature of 145o Fahr. On the other hand, with two leaves, one placed in water at 145o (62o.7 Cent.), and the other in water at 140o (60oCent.), both being left therein until the water cooled, the glands of both became white and porcelain-like. So that the duration of the immersion is an important element in the result.

Experiment 9.--A leaf was placed in water at 140o (60o Cent.), which was raised to 150o(65o.5 Cent.); there was no inflection; on the contrary, the outer tentacles were somewhat bowed backwards. The glands became like porcelain, but some of them were a little mottled with purple. The bases of the glands were often more affected than their summits. This leaf having been left in the strong solution did not undergo any inflection or aggregation.