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Weigh out
Methylene-blue 1.5 grammes
Place in a stoppered bottle of 150 c.c. capacity and add
Alcohol, 90 per cent 100.0 c.c.
Allow the alcohol to remain in contact with the dye for two hours, shaking vigourously every few minutes. Filter.
3. _Carbolic Methylene-blue_ (Kuehne).
Weigh out
Methylene-blue 1.5 grammes Carbolic acid 5.0 grammes
and dissolve in
Distilled water 100.0 c.c.
and add
Absolute alcohol 10.0 c.c.
Filter.
4. _Alkaline Methylene-blue_ (Loeffler).
Measure out and mix
Methylene-blue, saturated alcoholic solution 30.0 c.c.
Caustic potash, 0.1 per cent. aqueous solution 100.0 c.c.
Filter.
~Gentian Violet.~--
5. _Saturated Aqueous Solution._
Weigh out
Gentian violet 2.25 grammes
and proceed as in preparing the corresponding solution of methylene-blue.
6. _Saturated Alcoholic Solution._
Weigh out
Gentian violet 5.0 grammes
and proceed as in preparing the corresponding solution of methylene-blue.
7. _Carbolic Gentian Violet_ (Nicolle).
Measure out and mix
Gentian violet, saturated alcoholic solution 10.0 c.c.
Carbolic acid, 1 per cent. aqueous solution 100.0 c.c.
Filter.
8. _Anilin Water Solution_ (Koch-Ehrlich).
Measure out
Distilled water 100 c.c.
Add anilin oil drop by drop (shaking well after the addition of each drop) until the solution is opaque.
Filter until clear.
and add
Absolute alcohol 10 c.c.
Saturated alcoholic solution gentian violet 11 c.c.
Filter.
NOTE.--This solution will not keep longer than 14 days.
~Thionine Blue (or Lauth's Violet).~--
9. _Carbolic Thionine Blue_ (Nicolle).
Weigh out
Thionine blue 1.0 gramme Carbolic acid 2.5 grammes
and dissolve in
Distilled water 100.0 c.c.
Filter.
Before use dilute with equal quant.i.ty of distilled water and again filter.
~Fuchsin (Basic).~--
