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2. Colonies Confluent: Surface elevation and character of edge, as for plate cultivations (_vide_ page 263).
Chromogenicity: As for plate cultures.
~Gelatine Stab Cultures.~--
(A) _Surface Growth._--As for individual colonies in plate cultures (_vide_ page 261).
[Ill.u.s.tration: FIG. 150.--Stab cultivations--types of growth: a, Filiform; b, beaded; c, echinate; d, villous; e, arborescent.]
(B) _Line of Puncture._--
Filiform: Uniform growth, without special characters (Fig. 150, a).
Nodose: Consisting of closely aggregated colonies.
Beaded: Consisting of loosely placed or disjointed colonies (Fig. 150, b).
Papillate: Beset with papillate extensions.
Echinate: Beset with acicular extensions (Fig. 150, c).
Villous: Beset with short, undivided, hair-like extensions (Fig. 150, d).
Plumose: A delicate feathery growth.
[Ill.u.s.tration: FIG. 151.--Stab cultivations--types of growth: f, Crateriform; g, saccate; h, infundibuliform; j, napiform; k, fusiform; l, stratiform.]
Arborescent: Branched or tree-like, beset with branched hair-like extensions (Fig. 150, e).
(C) _Area of Liquefaction_ (if present).--
Crateriform: A saucer-shaped liquefaction of the gelatine (Fig. 151, f).
Saccate: Shape of an elongated sack, tubular cylindrical (Fig. 151, g).
Infundibuliform: Shape of a funnel, conical (Fig. 151, h).
Napiform: Shape of a turnip (Fig. 151, j).
Fusiform: Outline of a parsnip, narrow at either end, broadest below the surface (Fig. 151, k).
Stratiform: Liquefaction extending to the walls of the tube and downward horizontally (Fig. 151, l).
(D) _Character of the Liquefied Gelatine._--
1. Pellicle on surface.
2. Uniformly turbid.
3. Granular.
4. Mainly clear, but containing flocculi.
5. Deposit at apex of liquefied portion.
(E) _Production of Gas Bubbles._
~Shake Cultures.~--
1. Presence or absence of liquefaction.
2. Production of gas bubbles.
3. Bulk of growth at the surface--aerobic.
4. Bulk of growth in depths--anaerobic.
~Fluid Media.~
~1. Surface of the Liquid.~--
Presence or absence of froth due to gas bubbles.
Presence or absence of pellicle formation.
Character of pellicle.
~2. Body of the Liquid.~--
Uniformly turbid.
Flocculi in suspension.
Granules in suspension.
Clear, with precipitate at bottom of tube.
Colouration of fluid, presence or absence of.
~3. Precipitate.~--
